PAOX Polyklonaler Antikörper

PAOX Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 18972-1-AP

Synonyme

EC:1.5.3.13, PAO, Peroxisomal N(1)-acetyl-spermine/spermidine oxidase, UNQ1923/PRO4398

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  WB analysis using 18972-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18972-1-AP (PAOX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 18972-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 18972-1-AP (PAOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 18972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18972-1-AP (PAOX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18972-1-AP (PAOX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, humanes Magengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

18972-1-AP bindet in WB, IHC, IF, ELISA PAOX und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAOX fusion protein Ag5777
• Vollständiger Name: polyamine oxidase (exo-N4-amino)
• Berechnetes Molekulargewicht: 511 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC032778
• Gene symbol: PAOX
• Gene ID (NCBI): 196743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAOX, also known as PAO, belongs to the flavin monoamine oxidase family. PAOX is a FAD-dependent amine oxidase, which is constitutively expressed in nearly all tissues of the vertebrate organism. PAOX catalyzes the oxidation of N1-acetylspermine to spermidine and thus involved in the polyamine back-conversion. PAOX can also oxidize N1-acetylspermidine to putrescine. PAOX has 4 isoforms with the molecular weights of 7, 35, 52 and 56 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.; Authors - Leslie A Sandusky-Beltran
rat	IF	Invest Ophthalmol Vis Sci; Regulation of Spermine Oxidase through Hypoxia-Inducible Factor-1α Signaling in Retinal Glial Cells under Hypoxic Conditions.; Authors - Di Wu
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
human	WB	PNAS Nexus; Acidic extracellular pH drives accumulation of N1-acetylspermidine and recruitment of protumor neutrophils; Authors - Miki Kato
human	WB	Proc Natl Acad Sci U S A; Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancer; Authors - Xinlu Han
mouse,human	WB	Sci Rep; Identifying polyamine related biomarkers in diagnosis and treatment of ulcerative colitis by integrating bulk and single-cell sequencing data; Authors - Wanhui Wei