PANX2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PANX2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 26604-1-AP

Synonyme

hPANX2, pannexin 2, PANX2, PX2

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse lung using 26604-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 26604-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 26604-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26604-1-AP (PANX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26604-1-AP (PANX2 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Mausmilzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

26604-1-AP bindet in WB, IHC, ELISA PANX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PANX2 fusion protein Ag24256
• Vollständiger Name: pannexin 2
• Berechnetes Molekulargewicht: 677 aa, 74 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC101023
• Gene symbol: PANX2
• Gene ID (NCBI): 56666
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pannexins (Panx) are proteins homologous to the invertebrate gap junction proteins called innexins (Inx) and are traditionally described as transmembrane channels. Three distinct Panx paralogs (Panx1, Panx2 and Panx3) have been identified in vertebrates. Previous reports on Panx expression and functionality are focused primarily on Panx1 and Panx3 proteins. The exact function of Panx2 remains elusive. Catalog#26604-1-AP specially recognizes the 70 kDa Panx2.

Publikationen

Species	Application	Title
human	WB	Nan Fang Yi Ke Da Xue Xue Bao; [Down-regulation of pannexin 2 channel enhances cisplatin-induced apoptosis in testicular cancer I-10 cells].; Authors - Yanxue Yao ; KD Validated