PALMD Polyklonaler Antikörper

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• KD/KO Validated

PALMD Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16531-1-AP

Synonyme

C1orf11, PALMD, palmdelphin, PALML, Paralemnin like protein

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16531-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16531-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16531-1-AP

  IP Result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug.

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  IP experiment using 16531-1-AP

  IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate.

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16531-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mausherzgewebe,
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

16531-1-AP bindet in WB, IP, IHC, IF, ELISA PALMD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PALMD fusion protein Ag9796
• Vollständiger Name: palmdelphin
• Berechnetes Molekulargewicht: 551 aa, 63 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC012570
• Gene symbol: PALMD
• Gene ID (NCBI): 54873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	Cell Death Dis; Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage.; Authors - N Dashzeveg ; KD Validated
human	WB	Front Oncol; Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.; Authors - Min-Yun Cai ; KD Validated
human	WB,IF	bioRxiv; Role of a Pdlim5:PalmD complex in directing dendrite morphology; Authors - Yogesh Srivastava
mouse	IF,IHC	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu