PAK1 Polyklonaler Antikörper

PAK1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 51137-1-AP

Synonyme

Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 51137-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 51137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51137-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 51137-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 51137-1-AP

  Immunofluorescent analysis of HeLa cells using 51137-1-AP (PAK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C6-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

51137-1-AP bindet in WB, IHC, IF, ELISA PAK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 1
• Berechnetes Molekulargewicht: 553 aa, 62 kDa
• Beobachtetes Molekulargewicht: 61-65 kDa
• GenBank-Zugangsnummer: BC109299
• Gene symbol: PAK1
• Gene ID (NCBI): 5058
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

Publikationen

Species	Application	Title
human	IHC	Pathol Res Pract; Clinicopathological significance and the associated signaling pathway of p21-activated kinase 1 (PAK1) in colorectal cancer; Authors - Jung-Soo Pyo