PAIP2 Polyklonaler Antikörper

PAIP2 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15583-1-AP

Synonyme

PABP interacting protein 2, PAIP 2, PAIP2, PAIP2A

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Galerie der Validierungsdaten

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  WB analysis of A431 using 15583-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15583-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15583-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15583-1-AP (PAIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 15583-1-AP

  IP Result of anti-PAIP2 (IP:15583-1-AP, 4ug; Detection:15583-1-AP 1:500) with A431 cells lysate 1200ug.

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  IF Staining of HeLa using 15583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15583-1-AP (PAIP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

15583-1-AP bindet in WB, IP, IHC, IF, ELISA PAIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAIP2 fusion protein Ag7119
• Vollständiger Name: poly(A) binding protein interacting protein 2
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC001716
• Gene symbol: PAIP2
• Gene ID (NCBI): 51247
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IHC	J Neurosci; Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity.; Authors - Chenghai Dong
human	WB	Front Genet; PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration; Authors - Chenyu Wang