PAFAH1B3 Polyklonaler Antikörper

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• KD/KO Validated

PAFAH1B3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20564-1-AP

Synonyme

PAF AH 29 kDa subunit, PAF AH subunit gamma, PAFAH subunit gamma, PAFAH1B3, PAFAHG

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Galerie der Validierungsdaten

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  WB analysis of human brain using 20564-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20564-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20564-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 20564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 20564-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 20564-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

20564-1-AP bindet in WB, IHC, IF, ELISA PAFAH1B3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26-29 kDa
• GenBank-Zugangsnummer: NM_002573
• Gene symbol: PAFAH1B3
• Gene ID (NCBI): 5050
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAFAH1B3, also named as PAFAHG, belongs to the 'GDSL' lipolytic enzyme family and Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. It inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. PAFAH1B3 plays an important role during the development of brain. The antibody is specific to PAFAH1B3.

Publikationen

Species	Application	Title
human	WB,IHC	Onco Targets Ther; Aberrant expression of PAFAH1B3 associates with poor prognosis and affects proliferation and aggressiveness in hypopharyngeal squamous cell carcinoma.; Authors - Jianing Xu ; KD Validated
human	IHC,IF	Discov Oncol; Increased PAFAH1B3 was associated with poor prognosis and T-cell exhaustion microenvironment in hepatocellular carcinoma; Authors - Genhao Zhang