PADI4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PADI4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 17373-1-AP

Synonyme

HL 60 PAD, PAD, PAD4, PADI4, PADI5, PDI4, PDI5, Peptidylarginine deiminase IV

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Galerie der Validierungsdaten

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  WB analysis of Purified protein using 17373-1-AP

  WB result of PADI4 antibody (17373-1-AP) with purified protein by T.J. Hollingsworth, Ph.D., University of Tennessee Health Sciences Center.

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  WB analysis using 17373-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 17373-1-AP

  IP Result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 17373-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17373-1-AP (PADI4 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 17373-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 17373-1-AP (PADI4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 17373-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PADI4 (17373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HepG2-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 6 publications below
• IF: See 12 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

17373-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA PADI4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PADI4 fusion protein Ag11068
• Vollständiger Name: peptidyl arginine deiminase, type IV
• Berechnetes Molekulargewicht: 663 aa, 74 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC025718
• Gene symbol: PADI4
• Gene ID (NCBI): 23569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

Publikationen

Species	Application	Title
rat	WB	Int J Mol Sci; Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.; Authors - Szilvia Török
mouse	WB	RMD Open; IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.; Authors - Ayano Yahagi
human	WB, IF	Transl Stroke Res; Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.; Authors - Hanhai Zeng
mouse	WB	Virulence; Extracellular trap can be trained as a memory response.; Authors - Yu Gao
mouse	IF	FASEB J; Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.; Authors - Kiyomi Mizugishi
mouse	WB	Front Immunol; TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.; Authors - Xinyan Wu