PADI2 Monoklonaler Antikörper

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• KD/KO Validated

PADI2 Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

2D8C12

Kat-Nr. : 66386-1-Ig

Synonyme

KIAA0994, PAD H19, PAD2, PADI2, PADI2 protein, PDI 2, PDI2, Peptidylarginine deiminase II

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 66386-1-Ig

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.

• 

  WB analysis of human skeletal muscle using 66386-1-Ig

  human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66386-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66386-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66386-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66386-1-Ig

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells.

• 

  IHC staining of human breast cancer using 66386-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66386-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66386-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, Hausschwein-Hirngewebe, HeLa-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Rattenhirngewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66386-1-Ig bindet in WB, IHC, IF, ELISA PADI2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PADI2 fusion protein Ag17612
• Vollständiger Name: peptidyl arginine deiminase, type II
• Berechnetes Molekulargewicht: 665 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC009701
• Gene symbol: PADI2
• Gene ID (NCBI): 11240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Publikationen

Species	Application	Title
human	IHC	J Immunother Cancer; Protein citrullination as a source of cancer neoantigens.; Authors - Hiroyuki Katayama
mouse	IHC,WB	Cell Death Dis; Peptidylarginine deiminase 2 plays a key role in osteogenesis by enhancing RUNX2 stability through citrullination; Authors - Hyun-Jung Kim ; KD Validated
human,mouse	WB,IF	Cell Mol Life Sci; ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.; Authors - Hyun-Jung Kim ; KD Validated
rat	WB	Mol Cell Proteomics; Changes in the Oligodendrocyte Progenitor Cell Proteome with Ageing.; Authors - Alerie G de la Fuente
mouse	IF	Acta Pharm Sin B; Cholinergic dysfunction-induced insufficient activation of alpha7 nicotinic acetylcholine receptor drives the development of rheumatoid arthritis through promoting protein citrullination via the SP3/PAD4 pathway; Authors - Changjun Lv