PABPN1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PABPN1 Monoklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

3C12E12

Kat-Nr. : 66807-1-Ig

Synonyme

OPMD, PAB2, PABII, PABP 2, PABP2, PABPN1, Poly(A) binding protein 2, Poly(A) binding protein II

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Galerie der Validierungsdaten

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  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66807-1-Ig

  WB result of PABPN1 antibody (66807-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPN1 transfected HeLa cells.

• 

  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 66807-1-Ig

  IP result of anti-PABPN1 (IP:66807-1-Ig, 5ug; Detection:66807-1-Ig 1:600) with Jurkat cells lysate 1680 ug.

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  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, Mausherzgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

66807-1-Ig bindet in WB, RIP, IP, IHC, ELISA PABPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PABPN1 fusion protein Ag27470
• Vollständiger Name: poly(A) binding protein, nuclear 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC010939
• Gene symbol: PABPN1
• Gene ID (NCBI): 8106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).

Publikationen

Species	Application	Title
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
mouse	WB	J Biol Chem; Extracellular 5'-methylthioadenosine inhibits intracellular symmetric dimethylarginine protein methylation of FUSE-element binding proteins; Authors - Baiqing Tang
human	WB,IHC,RIP	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen ; KD Validated
human	WB	Nat Cell Biol; An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus; Authors - Albertas Navickas