PABPC4 Monoklonaler Antikörper

PABPC4 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6B3

Kat-Nr. : 68187-1-Ig

Synonyme

Activated platelet protein 1, APP 1, APP1, FLJ43938, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

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Galerie der Validierungsdaten

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  WB analysis of HSC-T6 using 68187-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 68187-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 68187-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 68187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68187-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 68187-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PABPC4 antibody (68187-1-Ig, Clone: 1E6B3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68187-1-Ig bindet in WB, IHC, IF, ELISA PABPC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PABPC4 fusion protein Ag6813
• Vollständiger Name: poly(A) binding protein, cytoplasmic 4 (inducible form)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC065540
• Gene symbol: PABPC4
• Gene ID (NCBI): 8761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Poly(A)-binding protein (PABP), a nucleocytoplasmic shuttling protein, has a fundamental role in promoting gene expression by enhancing mRNA translation and stability(PMID: 21940797). Poly(A)-binding protein 4 (PABPC4), one of the homologs of PABP, was initially identified as a human T-cell activation-induced protein. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules(NCBI).