PABPC4 Polyklonaler Antikörper

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• KD/KO Validated

PABPC4 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14960-1-AP

Synonyme

Activated platelet protein 1, APP 1, APP1, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

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Galerie der Validierungsdaten

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  WB analysis using 14960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14960-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14960-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14960-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14960-1-AP (PABPC4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14960-1-AP

  IP Result of anti-PABPC4 (IP:14960-1-AP, 4ug; Detection:14960-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human gliomas using 14960-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 14960-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14960-1-AP (PABPC4 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 14960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PABPC4 antibody (14960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14960-1-AP

  Immunofluorescent analysis of Hela cells, using PABPC4 antibody 14960-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14960-1-AP bindet in WB, IP, IHC, IF, ELISA PABPC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PABPC4 fusion protein Ag6813
• Vollständiger Name: poly(A) binding protein, cytoplasmic 4 (inducible form)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70-71 kDa
• GenBank-Zugangsnummer: BC065540
• Gene symbol: PABPC4
• Gene ID (NCBI): 8761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
human	WB	J Immunol; HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.; Authors - Adi Reches ; KD Validated
human	WB	BMC Pregnancy Childbirth; Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.; Authors - Xiaotong Sun
human	WB	PLoS Pathog; Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes; Authors - Emily Yang
mouse	IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
human	WB	Nat Cell Biol; An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus; Authors - Albertas Navickas