PAAF1 Polyklonaler Antikörper

PAAF1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17650-1-AP

Synonyme

PAAF, PAAF1, Protein G 16, Rpn14, WDR71

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 17650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17650-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17650-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17650-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 17650-1-AP

  Immunofluorescent analysis of HEK-293 cells using 17650-1-AP (PAAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17650-1-AP bindet in WB, IHC, IF, ELISA PAAF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAAF1 fusion protein Ag11892
• Vollständiger Name: proteasomal ATPase-associated factor 1
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC028628
• Gene symbol: PAAF1
• Gene ID (NCBI): 80227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Am J Cancer Res; PMID: 31285951; Authors - Wen-Si Zhao
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang