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P53 Monoklonaler Antikörper
P53 Monoklonal Antikörper für IF, IHC, WB, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte und mehr (4)
Anwendung
WB, IP, IHC, IF, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
6C4B6
Kat-Nr. : 60283-2-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Neuro-2a-Zellen, A375-Zellen, A431-Zellen, COLO 320-Zellen, HaCaT-Zellen, HEK-293-Zellen, HT-29-Zellen, Maus-Kolongewebe, Ratten-Thymusgewebe, ROS1728-Zellen |
| Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe, humanes Gliomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | A431-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunhistochemie (IHC) | IHC : 1:1600-1:6400 |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 5 publications below |
| WB | See 158 publications below |
| IHC | See 20 publications below |
| IF | See 19 publications below |
| IP | See 7 publications below |
| CoIP | See 3 publications below |
Produktinformation
60283-2-Ig bindet in WB, IP, IHC, IF, CoIP, ELISA P53 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Hund, Kaninchen, Ratte, Zebrafisch |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | P53 fusion protein Ag0698 |
| Vollständiger Name | tumor protein p53 |
| Berechnetes Molekulargewicht | 44 kDa |
| Beobachtetes Molekulargewicht | 53 kDa |
| GenBank-Zugangsnummer | BC003596 |
| Gene symbol | TP53 |
| Gene ID (NCBI) | 7157 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
1. What is p53?
P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.
2. FAQs and p53
a. I fail to detect p53 by western blotting
Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.
b. I fail to detect p53 in some cell lines by western blotting
Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.
c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size
p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for P53 antibody 60283-2-Ig | Protokoll herunterladen |
| IHC protocol for P53 antibody 60283-2-Ig | Protokoll herunterladen |
| IF protocol for P53 antibody 60283-2-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Bioact Mater Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma | ||
Redox Biol Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice | ||
Aging (Albany NY) Whole-transcriptome sequencing analysis reveal mechanisms of Yiqi Huoxue Yangyin (YHY) decoction in ameliorating D-gal-induced cardiac aging | ||
Redox Biol SSBP1 drives high fructose-induced glomerular podocyte ferroptosis via activating DNA-PK/p53 pathway. | ||
J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chiara (Verified Customer) (03-10-2023) | The antibody work well and was specific on the target
 |
FH Janelle (Verified Customer) (09-12-2022) | Specific to target
|
FH Allison (Verified Customer) (08-19-2019) | The antibody specifications online were easy to follow. I used the recommended dilution of antibody of 1:2000 which worked and could probably be diluted further.
|
