P4HA3 Polyklonaler Antikörper

P4HA3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 23185-1-AP

Synonyme

4 PH alpha 3, P4HA3

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Galerie der Validierungsdaten

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  WB analysis using 23185-1-AP

  Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23185-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23185-1-AP (P4HA3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of A549 using 23185-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using P4HA3 antibody (23185-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A549-Zellen
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

23185-1-AP bindet in WB, IHC, IF, ELISA P4HA3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P4HA3 fusion protein Ag19716
• Vollständiger Name: prolyl 4-hydroxylase, alpha polypeptide III
• Berechnetes Molekulargewicht: 544 aa, 61 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 61 kDa
• GenBank-Zugangsnummer: BC126170
• Gene symbol: P4HA3
• Gene ID (NCBI): 283208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

Publikationen

Species	Application	Title
mouse,rat	WB	J Mol Cell Cardiol; Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.; Authors - Randy T Cowling
human	WB	Commun Biol; TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.; Authors - Fumie Nakasuka ; KD Validated
mouse	IHC	J Dent Sci; Thrombospondin-1 and prolyl 4-hydroxylase subunit alpha 3 as potential biomarkers of salivary gland fibrosis; Authors - Zijian Zhang
human	WB	Mol Cell Biochem; Transcriptome sequencing identifies prognostic genes involved in gastric adenocarcinoma; Authors - Mingyue Li ; KD Validated