P4HA1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

P4HA1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1H10A6

Rezensionen (1)

Kat-Nr. : 66101-1-Ig

Synonyme

4 PH alpha 1, P4HA, P4HA1

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Galerie der Validierungsdaten

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  WB analysis using 66101-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66101-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66101-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66101-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66101-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66101-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 66101-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66101-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66101-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 66101-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:50.

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  IF Staining of PC-3 using 66101-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using 66101-1-Ig(P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, Rattenlebergewebe, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

66101-1-Ig bindet in WB, IHC, IF, ELISA P4HA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P4HA1 fusion protein Ag17867
• Vollständiger Name: prolyl 4-hydroxylase, alpha polypeptide I
• Berechnetes Molekulargewicht: 534 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC034998
• Gene symbol: P4HA1
• Gene ID (NCBI): 5033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.

Publikationen

Species	Application	Title
human	WB,IHC	Mol Oncol; Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.; Authors - Johanna Eriksson ; KD Validated
human	WB	Front Oncol; P4HA1 Regulates CD31 via COL6A1 in the Transition of Glioblastoma Stem-Like Cells to Tumor Endothelioid Cells.; Authors - Xiangming Han ; KD Validated
human	WB	Front Mol Biosci; Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.; Authors - Chenghao Zhanghuang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sumreet Singh (Verified Customer) (11-30-2018)

This antibody works very well for our data analysis and serves our purpose. It does not give any background and works great. Please send gift card from Amazon to this address:Accounts Payable Specialist Modern Meadow, Inc.340 Kingsland Street, Building 102Nutley, NJ 07110

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Purified protein