P2RX7 Polyklonaler Antikörper

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• KD/KO Validated

P2RX7 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 28207-1-AP

Synonyme

Homo sapiens (Human), P2RX7, P2X7

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Galerie der Validierungsdaten

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  WB analysis using 28207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28207-1-AP (P2RX7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

28207-1-AP bindet in WB, IHC, IF, ELISA P2RX7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX7 fusion protein Ag27700
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 7
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC011913
• Gene symbol: P2RX7
• Gene ID (NCBI): 5027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publikationen

Species	Application	Title
mouse,rat	WB	J Cell Physiol; Apelin-13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin-1/P2X7 axis and FAM134B-dependent reticulophagy.; Authors - Yiyuan Yang ; KD Validated
mouse,rat	WB,IHC,IF	Front Pharmacol; Salidroside Ameliorates Depression by Suppressing NLRP3-Mediated Pyroptosis via P2X7/NF-κB/NLRP3 Signaling Pathway.; Authors - Yuhui Chai
human	WB	Biochem Biophys Res Commun; A438079 affects colorectal cancer cell proliferation, migration, apoptosis, and pyroptosis by inhibiting the P2X7 receptor.; Authors - Ying Zhang
human	WB,IHC	Adv Med Sci; The role of P2X7 receptor in prognosis and metastasis of colorectal cancer.; Authors - Ying Zhang
human	WB	Biomed Res Int; Progesterone Reduces ATP-Induced Pyroptosis of SH-SY5Y Cells; Authors - Chang Cui
human,mouse	WB,IHC	Phytother Res; Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer; Authors - Xuemei Huang