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Occludin Polyklonaler Antikörper

Occludin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-27260

Synonyme

occludin, OCLN

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    IF Staining of MCF-7 using CL488-27260

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:800.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    IF Staining of Caco-2 using CL488-27260

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    FC experiment of MCF-7 using CL488-27260

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Occludin (CL488-27260) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMCF-7-Zellen, Caco-2-Zellen
Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:400-1:1600
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-27260 bindet in IF, FC (Intra) Occludin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Occludin fusion protein Ag26173
Vollständiger Name occludin
Berechnetes Molekulargewicht 522 aa, 59 kDa
GenBank-ZugangsnummerBC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Occludin antibody CL488-27260Protokoll herunterladen
FC protocol for CL Plus 488 Occludin antibody CL488-27260Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen