OXTR Polyklonaler Antikörper

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• KD/KO Validated

OXTR Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 23045-1-AP

Synonyme

OT R, OXTR, Oxytocin receptor

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 23045-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23045-1-AP (OXTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 23045-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23045-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 23045-1-AP

  IP Result of anti-OXTR (IP:23045-1-AP, 4ug; Detection:23045-1-AP 1:1000) with L02 cells lysate 1800ug.

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23045-1-AP (OXTR antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 23045-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 23045-1-AP (Oxytocin Receptor antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 23045-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug OXTR antibody (23045-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Uterusmyomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

23045-1-AP bindet in WB, IP, IHC, IF, FC, ELISA OXTR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXTR fusion protein Ag19074
• Vollständiger Name: OXTR
• Berechnetes Molekulargewicht: 389 aa, 43 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 67 kDa
• GenBank-Zugangsnummer: BC137443
• Gene symbol: OXTR
• Gene ID (NCBI): 5021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in endometrium, myometrium, mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; PMID: 24915299; Authors - Christian Elabd
mouse	IF	Acta Physiol (Oxf); Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.; Authors - Changcheng Sun
mouse	IF	Oxid Med Cell Longev; PMID: 32082478; Authors - Daniela C Wigger
mouse	IHC	Antioxidants (Basel); ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.; Authors - Britta Trautwein
human	WB	Biochem Pharmacol; Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.; Authors - Mengmeng Wang ; KD Validated
human	WB	Front Pharmacol; The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.; Authors - Chaolu Chen