OXA1L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OXA1L Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 21055-1-AP

Synonyme

Hsa, OXA1 like protein, OXA1Hs, OXA1L

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Galerie der Validierungsdaten

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  WB analysis using 21055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21055-1-AP

  WB result of OXA1L antibody (21055-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXA1L transfected HeLa cells.

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  WB analysis of NIH/3T3 using 21055-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21055-1-AP (OXA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OXA1L antibody (21055-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 21055-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 21055-1-AP (OXA1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, L02-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

21055-1-AP bindet in WB, IP, IF, ELISA OXA1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXA1L fusion protein Ag13819
• Vollständiger Name: oxidase (cytochrome c) assembly 1-like
• Berechnetes Molekulargewicht: 437 aa, 49 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC001669
• Gene symbol: OXA1L
• Gene ID (NCBI): 5018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OXA1L/Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family and is required for cytochrome c oxidase assembly. The OXA1L protein was initially identified in yeast proteins involved in the biogenesis of the mitochondrial respiratory chain complexes. It locates in the inner membrane of mitochondria and may be involved in mediating the insertion of proteins from the matrix into the inner membrane.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
human	WB,IP	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
human	WB	Am J Hum Genet; Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.; Authors - Kyle Thompson
human	WB	EMBO Mol Med; OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.; Authors - Kyle Thompson ; KD Validated
mouse	WB	Aging (Albany NY); Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation.; Authors - Kara L Perks
mouse	WB	J Cell Sci; Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis; Authors - Richard G. Lee

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot