OTUD7B Monoklonaler Antikörper

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• KD/KO Validated

OTUD7B Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

2C12D9

Kat-Nr. : 66276-1-Ig

Synonyme

CEZANNE, OTU domain containing 7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 66276-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66276-1-Ig (OTUD7B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66276-1-Ig bindet in WB, IHC, IF OTUD7B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OTUD7B fusion protein Ag10513
• Vollständiger Name: OTU domain containing 7B
• Berechnetes Molekulargewicht: 427aa,48 kDa; 843aa,93 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC020622
• Gene symbol: OTUD7B
• Gene ID (NCBI): 56957
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity.

Publikationen

Species	Application	Title
human	WB	Nature; TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling.; Authors - Bin Wang ; KD Validated
human	WB,IF	Autophagy; High-throughput screening of functional deubiquitinating enzymes in autophagy.; Authors - Shuo Tian ; KO Validated
human	WB,IF	Autophagy; OTUD7B deubiquitinates SQSTM1/p62 and promotes IRF3 degradation to regulate antiviral immunity.; Authors - Weihong Xie ; KD Validated
human	WB	Mol Biol Rep; DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells; Authors - Qi‑Zhou Zhu ; KD Validated
human	IHC	J Exp Clin Cancer Res; OTUD7B suppresses Smac mimetic-induced lung cancer cell invasion and migration via deubiquitinating TRAF3.; Authors - Boxiang Zhang
human	WB, IHC	Cell Death Dis; OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation.; Authors - Jianing Tang