OTC Polyklonaler Antikörper

OTC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 26470-1-AP

Synonyme

OCTD, ornithine carbamoyltransferase, Ornithine transcarbamylase, OTC, OTCase

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Galerie der Validierungsdaten

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  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26470-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26470-1-AP (OTC antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26470-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26470-1-AP (OTC antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below

Produktinformation

26470-1-AP bindet in WB, IHC, IF, ELISA OTC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OTC fusion protein Ag24186
• Vollständiger Name: ornithine carbamoyltransferase
• Berechnetes Molekulargewicht: 354 aa, 40 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC074745
• Gene symbol: OTC
• Gene ID (NCBI): 5009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine transcarbamylase (OTC) is a liver and intestinal mucosa-located, intramitochondrial, rate-limiting enzyme in the urea cycle. It catalyzes the reaction that converts ornithine and carbamoyl phosphate into citrulline, which is the second step of the urea cycle, to detoxify the ammonia produced from amino acid catabolism.

Publikationen

Species	Application	Title
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
human	WB	Front Cell Dev Biol; Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.; Authors - Changsen Bai
human	WB	Biochem Biophys Res Commun; Circadian oscillation expression of ornithine carbamoyltransferase and its significance in sleep disturbance.; Authors - Wei Wang
mouse	WB,IF	J Clin Invest; Glutamine synthetase limits b-catenin-mutated liver cancer growth by maintaining nitrogen homeostasis and suppressing mTORC1; Authors - Weiwei Dai
human	WB	Metab Brain Dis; p53 inhibits the Urea cycle and represses polyamine biosynthesis in glioma cell lines; Authors - Yuhong Zhao