ORAI3 Polyklonaler Antikörper

ORAI3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 25766-1-AP

Synonyme

ORAI3, Protein orai 3, TMEM142C, Transmembrane protein 142C

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 25766-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25766-1-AP (ORAI3 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human endometrial cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25766-1-AP (ORAI3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 25766-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25766-1-AP (ORAI3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25766-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ORAI3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORAI3 fusion protein Ag22513
• Vollständiger Name: ORAI calcium release-activated calcium modulator 3
• Berechnetes Molekulargewicht: 295 aa, 32 kDa
• Beobachtetes Molekulargewicht: ~30 kDa
• GenBank-Zugangsnummer: BC006126
• Gene symbol: ORAI3
• Gene ID (NCBI): 93129
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORAI3, also named as TMEM142C, is a key regulator or component of store-operated Ca2+ channel and transcription factor NFAT nuclear import. ORAI3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca2+ depotentiation. The MW of ORAI3 is about 30-32 kDa.

Publikationen

Species	Application	Title
human	WB,IF,CoIP	BMJ Open Diabetes Res Care; Orai-IGFBP3 signaling complex regulates high-glucose exposure-induced increased proliferation, permeability, and migration of human coronary artery endothelial cells.; Authors - Suwen Bai
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (02-06-2023)

Works pretty well for our WB

• Applications: Western Blot