ORAI2 Polyklonaler Antikörper

ORAI2 Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 20592-1-AP

Synonyme

C7orf19, CBCIP2, ORAI2, Protein orai 2, TMEM142B, Transmembrane protein 142B

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 20592-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20592-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20592-1-AP (ORAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20592-1-AP (ORAI2 Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 20592-1-AP

  Immunofluorescent analysis of HEK-293 cells using 20592-1-AP (ORAI2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HEK-293 using 20592-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug ORAI2 antibody (20592-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

20592-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ORAI2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORAI2 fusion protein Ag14540
• Vollständiger Name: ORAI calcium release-activated calcium modulator 2
• Berechnetes Molekulargewicht: 254 aa, 29 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC069270
• Gene symbol: ORAI2
• Gene ID (NCBI): 80228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ORAI2, also named as C7orf19, CBCIP2, TMEM142B and PP1729, is a Ca2+ release-activated Ca2+-like (CRAC-like) channel subunit. The MW of ORAI2 is about 36 kDa for glycosylation (PMID: 19675303).

Publikationen

Species	Application	Title
mouse	WB	J Am Heart Assoc; Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.; Authors - Jesse Gammons
rat	WB	Front Mol Biosci; X-Ray Causes mRNA Transcripts Change to Enhance Orai2-Mediated Ca2+ Influx in Rat Brain Microvascular Endothelial Cells.; Authors - Fangfang Xu
mouse	IP	BMJ Open Diabetes Res Care; Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.; Authors - Yuan Wei
human	WB	Exp Ther Med; Resveratrol sensitizes A549 cells to irradiation damage via suppression of store-operated calcium entry with Orai1 and STIM1 downregulation.; Authors - Wu Lele
mouse	WB	Phytochemistry; Bisbenzylisoquinoline alkaloids from Plumula Nelumbinis inhibit vascular smooth muscle cells migration and proliferation by regulating the ORAI2/Akt pathway; Authors - Xiao Feng
rat	WB	Allergy Asthma Immunol Res; Exercise Reduces Airway Smooth Muscle Contraction in Asthmatic Rats via Inhibition of IL-4 Secretion and Store-Operated Ca2+ Entry Pathway; Authors - Jun-Hao Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marcel (Verified Customer) (03-20-2023)

Works great.

• Applications: Western Blot
• Cell Tissue Type: Heart