ORAI1 Polyklonaler Antikörper

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• KD/KO Validated

ORAI1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 28411-1-AP

Synonyme

CRACM1, FLJ14466, ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, ORAT1, Protein orai 1, TMEM142A, Transmembrane protein 142A

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Galerie der Validierungsdaten

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  WB analysis using 28411-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 28411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28411-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

28411-1-AP bindet in WB, IHC, IF ORAI1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ORAI1 fusion protein Ag29234
• Vollständiger Name: ORAI calcium release-activated calcium modulator 1
• Berechnetes Molekulargewicht: 301 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC015369
• Gene symbol: ORAI1
• Gene ID (NCBI): 84876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. ORAI1 is a complex glycosylated protein, it exists in several molecular weight forms: a theoretical molecular weight of 32.7 kDa of the unmodified protein and two major glycosylated proteins of ~43 kDa and ~50 kDa (PMID: 22318387). Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene.

Publikationen

Species	Application	Title
human	WB	Front Oncol; Calcium Channel Protein ORAI1 Mediates TGF-β Induced Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells.; Authors - Qingjie Kang ; KD Validated
human	WB	J Mol Med (Berl); Phosphate-induced ORAI1 expression and store-operated Ca2+ entry in aortic smooth muscle cells.; Authors - Ke Ma
mouse	WB,IF	Life Sci; The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model.; Authors - Cheng-Shun Zhang
bovine	WB	J Dairy Sci; Role of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil extracellular trap formation in dairy cows with subclinical hypocalcemia.; Authors - Bingbing Zhang ; KD Validated
bovine	IF	J Anim Sci; Intracellular Ca2+ signaling and ORAI calcium release-activated calcium modulator 1 (ORAI1) are associated with hepatic lipidosis in dairy cattle.; Authors - Ming Li