OPA1 Monoklonaler Antikörper

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• KD/KO Validated

OPA1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

1B2D8

Kat-Nr. : 66583-1-Ig

Synonyme

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Galerie der Validierungsdaten

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66583-1-Ig

  WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 1 publications below

Produktinformation

66583-1-Ig bindet in WB, IHC, IF, ELISA OPA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OPA1 fusion protein Ag26868
• Vollständiger Name: optic atrophy 1 (autosomal dominant)
• Berechnetes Molekulargewicht: 960 aa, 112 kDa
• Beobachtetes Molekulargewicht: 100 kDa and 80-90 kDa
• GenBank-Zugangsnummer: BC075805
• Gene symbol: OPA1
• Gene ID (NCBI): 4976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
fish	WB	J Physiol Sci; Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart; Authors - Zhanglin Chen
	WB	Behav Brain Res; Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice.; Authors - Donghyun Kim
mouse	WB	Eur J Pharmacol; Dihydromyricetin contributes to weight loss via pro-browning mediated by mitochondrial fission in white adipose; Authors - Xiaowei Xiong
human	WB	Cell Death Dis; Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy; Authors - Lingna Yi
rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang