OPA1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OPA1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

103

Rezensionen (1)

Kat-Nr. : 27733-1-AP

Synonyme

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Galerie der Validierungsdaten

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  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27733-1-AP

  WB result of OPA1 antibody (27733-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27733-1-AP

  IP result of anti-OPA1(IP:27733-1-AP, 4ug; Detection:27733-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 98 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

27733-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA OPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, hamster, Hausschwein, Huhn, Maus, Ratte, Rind, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OPA1 fusion protein Ag26887
• Vollständiger Name: optic atrophy 1 (autosomal dominant)
• Berechnetes Molekulargewicht: 960 aa, 112 kDa
• Beobachtetes Molekulargewicht: 100 kDa, 80-90 kDa
• GenBank-Zugangsnummer: BC075805
• Gene symbol: OPA1
• Gene ID (NCBI): 4976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publikationen

Species	Application	Title
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
human	WB	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

Works well in human adipocyte cells, we got clean bands at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes