OLIG2 Monoklonaler Antikörper

OLIG2 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8C5

Kat-Nr. : CL488-66513

Synonyme

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

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Galerie der Validierungsdaten

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  IF Staining of mouse brain using CL488-66513

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 OLIG2 antibody (CL488-66513, Clone: 1E8C5 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL488-66513

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 OLIG2 antibody (CL488-66513, Clone: 1E8C5 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66513 bindet in IF OLIG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: OLIG2 fusion protein Ag18838
• Vollständiger Name: oligodendrocyte lineage transcription factor 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35-39 kDa
• GenBank-Zugangsnummer: BC047511
• Gene symbol: OLIG2
• Gene ID (NCBI): 10215
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )