hIST1 Polyklonaler Antikörper

hIST1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19842-1-AP

Synonyme

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

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Galerie der Validierungsdaten

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  WB analysis using 19842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19842-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19842-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19842-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19842-1-AP (hIST1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19842-1-AP

  IP Result of anti-hIST1 (IP:19842-1-AP, 4ug; Detection:19842-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 19842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19842-1-AP (hIST1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 19842-1-AP

  Immunofluorescent analysis of HepG2 cells using 19842-1-AP (hIST1 antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 4 publications below

Produktinformation

19842-1-AP bindet in WB, IHC, IF, ELISA hIST1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: hIST1 fusion protein Ag13874
• Vollständiger Name: KIAA0174
• Berechnetes Molekulargewicht: 364 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC000116
• Gene symbol: KIAA0174
• Gene ID (NCBI): 9798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human		MBio; Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.; Authors - Ekansh Mittal
human,mouse	IF	Cell Rep; Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation; Authors - Marine Gros
human	IF	bioRxiv; IST1 regulates select endosomal recycling pathways; Authors - Amy K Clippinger
human	IF	Traffic; IST1 regulates select recycling pathways; Authors - Amy K Clippinger