GTPBP9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GTPBP9 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (2)

Kat-Nr. : 16371-1-AP

Synonyme

GTP binding protein 9, GTPBP9, Obg like ATPase 1, OLA1, PRO2455, PTD004

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 16371-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16371-1-AP

  WB result of GTPBP9 antibody (16371-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GTPBP9 transfected HepG2 cells.

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  WB analysis of HepG2 using 16371-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

16371-1-AP bindet in WB, IHC, IF, ELISA GTPBP9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GTPBP9 fusion protein Ag9283
• Vollständiger Name: Obg-like ATPase 1
• Berechnetes Molekulargewicht: 396aa,45 kDa; 278aa,31 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC013925
• Gene symbol: OLA1
• Gene ID (NCBI): 29789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Clin Exp Pharmacol Physiol; OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.; Authors - Gayathri Narasimhan ; KD Validated
human,mouse	WB	medRxiv; Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart; Authors - Praveen K Dubey

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (03-20-2023)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

GTPBP9 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16371-1-AP)

FH

p (Verified Customer) (05-27-2021)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: H9C2

GTPBP9 Antibody Western Blot validation (1:3000 dilution) in H9C2 (Cat no:16371-1-AP)