OGDH Polyklonaler Antikörper

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• KD/KO Validated

OGDH Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

56

Kat-Nr. : 15212-1-AP

Synonyme

AKGDH, E1k, OGDC, OGDC E1, OGDH

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Galerie der Validierungsdaten

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  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 15212-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15212-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15212-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 15212-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15212-1-AP (OGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15212-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15212-1-AP (OGDH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells stained for OGDH rabbit pAb (15212-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15212-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG (H+L) for 66031-1-Ig. .

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  IF Staining of A549 using 15212-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using OGDH antibody (15212-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15212-1-AP

  Immunofluorescent analysis of Hela cells, using OGDH antibody 15212-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, A549-Zellen, Mauslebergewebe, Mauslungengewebe, Maus-Pankreasgewebe, Rattenherzgewebe, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 53 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

15212-1-AP bindet in WB, IP, IHC, IF, ELISA OGDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OGDH fusion protein Ag7368
• Vollständiger Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Berechnetes Molekulargewicht: 116 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC004964
• Gene symbol: OGDH
• Gene ID (NCBI): 4967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Nature; α-Ketoglutarate links p53 to cell fate during tumour suppression.; Authors - John P Morris ; KD Validated
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
human,mouse	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai