ODC1 Polyklonaler Antikörper

ODC1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23454-1-AP

Synonyme

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie der Validierungsdaten

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  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23454-1-AP bindet in WB, IHC, ELISA ODC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ODC1 fusion protein Ag10927
• Vollständiger Name: ornithine decarboxylase 1
• Berechnetes Molekulargewicht: 461 aa, 51 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC025296
• Gene symbol: ODC1
• Gene ID (NCBI): 4953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.