OBFC2B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OBFC2B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14809-1-AP

Synonyme

hSSB1, OBFC2B, Sensor of ssDNA subunit B1, SOSS B1, SOSS complex subunit B1, SSB1

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Galerie der Validierungsdaten

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  WB analysis using 14809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14809-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14809-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14809-1-AP

  IP Result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 14809-1-AP

  IP Result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 14809-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14809-1-AP

  Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

14809-1-AP bindet in WB, IP, IHC, IF, ELISA OBFC2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OBFC2B fusion protein Ag6532
• Vollständiger Name: oligonucleotide/oligosaccharide-binding fold containing 2B
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC001079
• Gene symbol: OBFC2B
• Gene ID (NCBI): 79035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
human	WB	Cell Death Dis; Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.; Authors - Anaïs Aulas
human	WB,IHC	J Inflamm Res; Comprehensive Analysis of NABP2 as a Prognostic Biomarker and Its Correlation with Immune Infiltration in Hepatocellular Carcinoma; Authors - Bowen Li