OBFC2A Polyklonaler Antikörper

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• KD/KO Validated

OBFC2A Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16719-1-AP

Synonyme

hSSB2, OBFC2A, Sensor of ssDNA subunit B2, SOSS B2, SOSS complex subunit B2, SSB2

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Galerie der Validierungsdaten

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  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

16719-1-AP bindet in WB, IP OBFC2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OBFC2A fusion protein Ag10158
• Vollständiger Name: oligonucleotide/oligosaccharide-binding fold containing 2A
• Berechnetes Molekulargewicht: 124 aa, 14 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC107723
• Gene symbol: OBFC2A
• Gene ID (NCBI): 64859
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].

Publikationen

Species	Application	Title
mouse	WB	Blood; Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress.; Authors - Wei Shi
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
mouse	WB	EMBO J; The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo.; Authors - Feldhahn Niklas N ; KD Validated
human	WB	Front Oncol; The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer.; Authors - Qianxue Wu
mouse	WB	J Immunol; SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.; Authors - Matthias Pfeifer ; KO Validated
human,mouse	IP	Sci Rep; hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage.; Authors - Didier Boucher