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NeutraControl Noggin Monoklonaler Antikörper

NeutraControl Noggin Monoklonal Antikörper für Non-Neutralization, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

Non-Neutralization, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10F3

Kat-Nr. : 69510-1-Ig

Synonyme

NOG, noggin, SYM1, SYNS1

Galerie der Validierungsdaten

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  • Non-Neutralization experiment of ATDC5 using NeutraControl Noggin Monoclonal antibody (69510-1-Ig)

    Non-Neutralization experiment of ATDC5 using 69510-1-Ig

    Recombinant human Noggin (Cat.NO. HZ-1118) inhibits BMP-4 induced alkaline phosphate production (using pNPP as chromogenic substrate for detection) by ATDC5 in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity of human Noggin (40 ng/mL HZ-1118) is neutralized by mouse anti-human Noggin monoclonal antibody 69010-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 5-20 μg/mL. The NeutraControl mouse anti-human Noggin monoclonal antibody 69510-1-Ig, generated from the same immunogen, could not neutralize human Noggin at the range of 0.0244-200 μg/mL(green curve, refer to top X-right Y).

  • ELISA experiment of Recombinant protein using NeutraControl Noggin Monoclonal antibody (69510-1-Ig)

    ELISA experiment of Recombinant protein using 69510-1-Ig

    Indirect ELISA was carried out by coating recombinant Human Noggin (Cat.NO. HZ-1118) at 70 ng/well followed by blocking and adding serial diluted Noggin antibody 69010-1-Ig and 69510-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

Produktinformation

69510-1-Ig bindet in Non-Neutralization, ELISA Noggin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen human Humankine Noggin protein HZ-1118
Vollständiger Name noggin
Gene symbol NOG
Gene ID (NCBI) 9241
Konjugation Unkonjugiert
Form Lyophilized Powder
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer Sterile PBS.
Endotoxin<0.1 EU/μg
WiederherstellungThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

This antibody is a neutralizing control antibody for Noggin, the immunogen is the same as Neutrakine 69010-1-Ig but could not neutralize human Noggin.