NeutraKine® PDGF-BB Monoklonaler Antikörper

NeutraKine® PDGF-BB Monoklonal Antikörper für Neutralization, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Neutralization, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4G11C7

Kat-Nr. : 69020-1-Ig

Synonyme

Becaplermin, c sis, FLJ12858, NeutraKine® PDGF-BB, PDGF 2, PDGF subunit B, PDGF2, PDGFB, PDGF-BB, Proto oncogene c Sis, SIS, SSV

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Galerie der Validierungsdaten

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  Neutralization experiment of NeutraKine® PDGF-BB using 69020-1-Ig

  Recombinant human PDGFbb (Cat.NO. HZ-1308) stimulates proliferation of BALB/3T3 (mouse fibroblast cell line) in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGFbb (20ng/mL HZ-1308) is neutralized by mouse anti-human PDGFbb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL.

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  Neutralization experiment of NeutraKine® PDGF-BB using 69020-1-Ig

  Recombinant human PDGF bb (Cat.NO. HZ-1308) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human PDGF bb (20 ng/mL HZ-1308) is neutralized by mouse anti-human PDGF bb monoclonal antibody 69020-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 20-50 μg/mL. The NeutraControl mouse anti-human PDGF bb monoclonal antibody 69520-1-Ig could recognize human PDGF bb, but could not neutralize human PDGF bb at the range of 3.125-200 μg/mL(green curve, refer to top X-right Y axis).

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  ELISA experiment of Recombinant protein using 69020-1-Ig

  Indirect ELISA was carried out by coating recombinant Human PDGF bb (Cat.NO. HZ-1308) at 70 ng/well followed by blocking and adding serial diluted PDGF bb antibody 69020-1-Ig and 69520-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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• Neutralization: BALB/3T3

Veröffentlichte Anwendungen

• ELISA: See 1 publications below

Produktinformation

69020-1-Ig bindet in Neutralization, Cell treatment, ELISA NeutraKine® PDGF-BB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human Humankine PDGF-BB protein HZ-1308
• Vollständiger Name: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• Gene symbol: PDGFB
• Gene ID (NCBI): 5155
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.

This antibody can be used to neutralize the bioactivity of PDGF-BB.

Publikationen

Species	Application	Title
human	ELISA	Free Radic Biol Med; IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis; Authors - Yao Zhang
human	Cell treatment	Metabolism; Hepatocyte Ninjurin2 promotes hepatic stellate cell activation and liver fibrosis through the IGF1R/EGR1/PDGF-BB signaling pathway; Authors - Yifan Wang

Verwandte Produkte

NeutraControl PDGF-BB Monoclonal antibody

69520-1-Ig