NeutraKine® IFN Gamma Monoklonaler Antikörper

NeutraKine® IFN Gamma Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

1E10G7

Kat-Nr. : CL555-69007

Synonyme

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

---

Galerie der Validierungsdaten

• 

  FC experiment of Jurkat using CL555-69007

  1X10^6 PMA and ionomycin treated Jurkat cells were intracellularly stained with 0.25 ug CoraLite®555 Anti-Human NeutraKine® IFN Gamma (CL555-69007, Clone:1E10G7), and 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated Jurkat cells

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-69007 bindet in FC (Intra) NeutraKine® IFN Gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeutraKine® IFN Gamma fusion protein HZ-1301
• Vollständiger Name: IFN gamma
• Gene symbol: IFNG
• Gene ID (NCBI): 3458
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

This antibody can be used to neutralize the bioactivity of Interferon gamma.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C