NeuN Monoklonaler Antikörper

NeuN Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

1

CloneNo.

3A4C1

Kat-Nr. : CL488-66836

Synonyme

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

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Galerie der Validierungsdaten

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  IF Staining of mouse brain using CL488-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66836 (NeuN antibody) at dilution of 1:100.

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  IF Staining of mouse cerebellum using CL488-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 NeuN antibody (CL488-66836, Clone: 3A4C1 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66836 (NeuN antibody), at dilution of 1:100.

• 

  FC experiment of U-87 MG using CL488-66836

  1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of SH-SY5Y using CL488-66836

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NeuN (CL488-66836, Clone:3A4C1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in FC: U-87 MG-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-66836 bindet in IF, FC (Intra) NeuN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag28016
• Vollständiger Name: hexaribonucleotide binding protein 3
• GenBank-Zugangsnummer: NM_001082575
• Gene symbol: hCG_1776007
• Gene ID (NCBI): 146713
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Publikationen

Species	Application	Title
rat	IF	Exp Eye Res; Geranylgeranylacetone-induced heat shock protein70 expression reduces retinal ischemia-reperfusion injury through PI3K/AKT/mTOR signaling; Authors - Lirong Zhang

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011