NeuN Monoklonaler Antikörper

NeuN Monoklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A4C1

Kat-Nr. : 66836-1-Ig

Synonyme

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3



Geprüfte Anwendungen

Erfolgreiche Detektion in IHCRatten-Cerebellum-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFMaus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe
Erfolgreiche Detektion in FCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 4 publications below
IFSee 58 publications below

Produktinformation

66836-1-Ig bindet in IHC, IF, FC, ELISA NeuN und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen NeuN fusion protein Ag28016
Vollständiger Name hexaribonucleotide binding protein 3
GenBank-ZugangsnummerNM_001082575
Gene symbol hCG_1776007
Gene ID (NCBI) 146713
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Protokolle

Produktspezifische Protokolle
IHC protocol for NeuN antibody 66836-1-IgProtokoll herunterladen
IF protocol for NeuN antibody 66836-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

Microbiome

The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.

Authors - Weiping Xiao
mouseIF

Cell Death Dis

Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

Authors - Yang Deng
ratIF

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang
mouseIF

Environ Int

Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis.

Authors - Diqi Yang
mouseIF

Elife

Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain

Authors - Shahzad S Khan
ratIF

Antioxidants (Basel)

Epimedium Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3-Mediated Pyroptosis

Authors - Xiaoyu Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenzo (Verified Customer) (01-05-2024)

This monoclonal NeuN worked well for mouse tissues.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Retina
FH

Silvia (Verified Customer) (08-11-2022)

it works well for Immunofluorescence on mature neurons

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: hiPSCs-derived neurons
FH

Delphine (Verified Customer) (07-25-2022)

Immunohistochemistry with the NeuN antibody on frozen spinal cord worked but the labeling must be optimized because there is a lot of background noise.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: tissue
FH

q (Verified Customer) (01-05-2022)

It is OK to use it in WB, but several non-specific bands above the expected MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
NeuN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66836-1-Ig)