Nestin Polyklonaler Antikörper

Nestin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-19483

Synonyme

FLJ21841, Nbla00170, NES, nestin

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Galerie der Validierungsdaten

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  IF Staining of human kidney using CL488-19483

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 Nestin antibody (CL488-19483) at dilution of 1:200.

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  FC experiment of neuronal cells derived from human dental pulp stem using CL488-19483

  Neuronal cells derived from human dental pulp stem cells were fixed in 70% ethanol and blocked in 0.1% BSA in Staining Buffer, then stained with CoraLite® Plus 488-conjugated Nestin Polyclonal Antibody (CL488-19483) (1:200) (red) or unstained (blue). Image credit: @stemcell_art on Instagram

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Nierengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-19483 bindet in IF, FC (Intra) Nestin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nestin
• Berechnetes Molekulargewicht: 177 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.