NXF1 Monoklonaler Antikörper

NXF1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C2H1

Kat-Nr. : CL488-67914

Synonyme

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

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Galerie der Validierungsdaten

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  IF Staining of HEK-293 using CL488-67914

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 NXF1 antibody (CL488-67914, Clone: 2C2H1 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL488-67914

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NXF1 (CL488-67914, Clone:2C2H1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67914 bindet in IF, FC (Intra) NXF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NXF1 fusion protein Ag27329
• Vollständiger Name: nuclear RNA export factor 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC004904
• Gene symbol: NXF1
• Gene ID (NCBI): 10482
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.