NVL Polyklonaler Antikörper

NVL Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 16970-1-AP

Synonyme

nuclear VCP like, Nuclear VCP like protein, NVL, NVLp

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Galerie der Validierungsdaten

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  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16970-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16970-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 16970-1-AP

  Immunofluorescent analysis of HepG2 cells using 16970-1-AP (NVL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, Mauslungengewebe, Mausnierengewebe, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

16970-1-AP bindet in WB, IF, ELISA NVL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NVL fusion protein Ag9205
• Vollständiger Name: nuclear VCP-like
• Berechnetes Molekulargewicht: 856aa,95 kDa; 659aa,73 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC012105
• Gene symbol: NVL
• Gene ID (NCBI): 4931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
human	IF	J Cell Biol; Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.; Authors - Yun Chawon C
human	WB,IF	Nat Commun; Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins; Authors - Arthur Louche

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (04-05-2020)

This antibody works okay. There are a few nonspecific bands, but I could tell which is the target band because I performed nuclear cytosolic fractionation. NVL is supposed to be in both nucleus and cytosol to shuttle ribosomes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

NVL Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16970-1-AP)