NUP153 Polyklonaler Antikörper

NUP153 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14189-1-AP

Synonyme

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153, NUP153

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14189-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14189-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14189-1-AP

  IP Result of anti-NUP153 (IP:14189-1-AP, 4ug; Detection:14189-1-AP 1:500) with K-562 cells lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

14189-1-AP bindet in WB, IP, ELISA NUP153 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP153 fusion protein Ag5519
• Vollständiger Name: nucleoporin 153kDa
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 154 kDa
• GenBank-Zugangsnummer: BC052965
• Gene symbol: NUP153
• Gene ID (NCBI): 9972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

Publikationen

Species	Application	Title
human	WB	Biotechnol J; Highly efficient identification of nucleocytoplasmic O-glycosylation by the TurboID-based proximity labeling method in living cells; Authors - Yunfeng Long