NUMB Monoklonaler Antikörper

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• KD/KO Validated

NUMB Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

CloneNo.

2G12B7

Kat-Nr. : 60137-1-Ig

Synonyme

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Galerie der Validierungsdaten

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  WB analysis of human brain using 60137-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 60137-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60137-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 60137-1-Ig

  Immunofluorescent analysis of A431 cells using 60137-1-Ig (NUMB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe,
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

60137-1-Ig bindet in WB, IHC, IF, CoIP, ELISA NUMB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: numb homolog (Drosophila)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC068476
• Gene symbol: NUMB
• Gene ID (NCBI): 8650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publikationen

Species	Application	Title
rat	WB,IF	Sci Rep; Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis.; Authors - Wen Xu ; KD Validated
mouse	WB,IF,CoIP	Neurosci Lett; Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors.; Authors - Na Wang ; KO Validated
human	WB	Mol Biol Rep; Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5.; Authors - Na Wang
rat	WB	Stem Cell Res Ther; BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction; Authors - Yan-Nan Xu
human	IF	Cell Rep; Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas; Authors - Nick A Kuburich