NUMB Polyklonaler Antikörper

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• KD/KO Validated

NUMB Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 18701-1-AP

Synonyme

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Galerie der Validierungsdaten

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  WB analysis using 18701-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18701-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 18701-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug NUMB antibody (18701-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

18701-1-AP bindet in WB, IHC, FC, ELISA NUMB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: numb homolog (Drosophila)
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 66-72 kDa
• GenBank-Zugangsnummer: BC068476
• Gene symbol: NUMB
• Gene ID (NCBI): 8650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publikationen

Species	Application	Title
human	WB	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
Human	IHC	Front Cell Dev Biol; IFT20 Mediates the Transport of Cell Migration Regulators From the Trans-Golgi Network to the Plasma Membrane in Breast Cancer Cells.; Authors - Huihui Yang
human	WB	Neurobiol Dis; The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.; Authors - Miao Sun ; KD Validated
human	WB,IHC	J Cell Mol Med; Musashi2 predicts poor prognosis and invasion in hepatocellular carcinoma by driving epithelial-mesenchymal transition.; Authors - Lu He
human	WB	Oncol Lett; A gene mutation in RNA-binding protein 10 is associated with lung adenocarcinoma progression and poor prognosis.; Authors - Lin-Lin Yin