NUDT21 Monoklonaler Antikörper

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• KD/KO Validated

NUDT21 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1C4C8

Kat-Nr. : 66335-1-Ig

Synonyme

CFIM25, CPSF 25 kDa subunit, CPSF25, CPSF5, Nudix motif 21, NUDT21

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 66335-1-Ig

  WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells.

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  WB analysis of NIH/3T3 using 66335-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66335-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66335-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66335-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66335-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66335-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 66335-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66335-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Produktinformation

66335-1-Ig bindet in WB, RIP, IP, IHC, IF, ELISA NUDT21 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NUDT21 fusion protein Ag12260
• Vollständiger Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC001403
• Gene symbol: NUDT21
• Gene ID (NCBI): 11051
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.

Publikationen

Species	Application	Title
human	IP	Mol Cell; A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.; Authors - Seung Wook Yang
human	WB,IF,IP,RIP	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	J Mol Cell Biol; U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors; Authors - Zhijie Hu