ARK5 Polyklonaler Antikörper

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• KD/KO Validated

ARK5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 22723-1-AP

Synonyme

AMPK related protein kinase 5, ARK5, KIAA0537, NUAK family SNF1 like kinase 1, NUAK1

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Galerie der Validierungsdaten

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  WB analysis using 22723-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22723-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

22723-1-AP bindet in WB, IHC, IF, ELISA ARK5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARK5 fusion protein Ag18612
• Vollständiger Name: NUAK family, SNF1-like kinase, 1
• Berechnetes Molekulargewicht: 661 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC152462
• Gene symbol: NUAK1
• Gene ID (NCBI): 9891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	PLoS Genet; A Mouse Model Uncovers LKB1 as an UVB-Induced DNA Damage Sensor Mediating CDKN1A (p21WAF1/CIP1) Degradation.; Authors - Rosaura Esteve-Puig ; KD Validated
human	IHC	Front Immunol; Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer; Authors - Yanling Zhou ; KD Validated
human	WB	Biomed Pharmacother; Discovery of a novel NUAK1 inhibitor against pancreatic cancer.; Authors - Myeong-Seong Seo
human	IHC	Int J Biol Sci; miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5.; Authors - Jingbang Wu
mouse	IF	Sci Rep; High-throughput kinase inhibitor screening reveals roles for Aurora and Nuak kinases in neurite initiation and dendritic branching.; Authors - Sara M Blazejewski
human	WB	Int J Immunopathol Pharmacol; Cancer susceptibility 18 positively regulates NUAK Family Kinase 1 expression to promote migration and invasion via sponging of miR-5586-5p in cervical cancer cells; Authors - Jingrong Wang