NTN1-Specific Polyklonaler Antikörper

NTN1-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20235-1-AP

Synonyme

netrin 1, NTN1, NTN1L, NTN1-Specific

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Galerie der Validierungsdaten

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  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20235-1-AP (NTN1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20235-1-AP (NTN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maushirngewebe, Rattenherzgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

20235-1-AP bindet in WB, IHC, ELISA NTN1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: netrin 1
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: NM_004822
• Gene symbol: NTN1
• Gene ID (NCBI): 9423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NTN1 is also named as NTN1L. Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1.

Publikationen

Species	Application	Title
human	WB	J Diabetes Res; High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR; Authors - Chenxiao Liu
human	WB	Int J Oncol; Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer.; Authors - Xiayun Jin
human	WB,IHC	J Int Med Res; Detection of netrin-1 as a novel biomarker for diagnosis and chemotherapeutic monitoring of lung cancer.; Authors - Yuanyuan Zhao
human,mouse	WB	Tissue Cell; Exosomes derived from human umbilical cord mesenchymal stem cells loaded with RVG-Lamp2b and Netrin-1 promotes Schwann cell invasion and migration; Authors - Jie He