NSUN6 Polyklonaler Antikörper

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• KD/KO Validated

NSUN6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 17240-1-AP

Synonyme

4933414E04Rik, NOPD1, NSUN6

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Galerie der Validierungsdaten

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  WB analysis using 17240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17240-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17240-1-AP (NSUN6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17240-1-AP

  WB result of NSUN6 antibody (17240-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN6 transfected HepG2 cells.

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  IHC staining of human colon cancer using 17240-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 17240-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17240-1-AP (NSUN6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

17240-1-AP bindet in WB, RIP, IHC, IF, ELISA NSUN6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSUN6 fusion protein Ag11084
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 6
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC035778
• Gene symbol: NSUN6
• Gene ID (NCBI): 221078
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.; Authors - Tommaso Selmi ; KO Validated
human	WB	PLoS Biol; Cytosine-5 RNA methylation links protein synthesis to cell metabolism.; Authors - Nikoletta A Gkatza
human	WB	Mol Syst Biol; CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.; Authors - Liang Fang ; KD Validated
human,mouse	WB,IHC,RIP	Exp Ther Med; Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m5C methylation; Authors - Sang Hu ; KD Validated
human	IHC,IF	J Cell Mol Med; RNA m5C methylation orchestrates BLCA progression via macrophage reprogramming; Authors - Dali Yan ; KO Validated
human	WB	Nat Struct Mol Biol; Genome-wide identification of mRNA 5-methylcytosine in mammals.; Authors - Tao Huang ; KD Validated