NSUN5 Polyklonaler Antikörper

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• KD/KO Validated

NSUN5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 15449-1-AP

Synonyme

NOL1, NOL1 related protein, NOL1R, NSUN5, NSUN5A, p120, WBSCR20, WBSCR20A, Ynl022cL

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15449-1-AP

  WB result of NSUN5 antibody (15449-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN5 transfected HeLa cells.

• 

  WB analysis of human kidney using 15449-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15449-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15449-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 15449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, humanes Nierengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Hautkrebsgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

15449-1-AP bindet in WB, IHC, IF, ELISA NSUN5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSUN5 fusion protein Ag7711
• Vollständiger Name: NOL1/NOP2/Sun domain family, member 5
• Berechnetes Molekulargewicht: 46.6 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC008084
• Gene symbol: NSUN5
• Gene ID (NCBI): 55695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.; Authors - Wei Dai ; KD Validated
mouse	WB,IF	Glia; Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.; Authors - Tingting Zhang
mouse	IF	Cells; Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.; Authors - Zihao Yuan ; KO Validated
human	IHC	Front Oncol; Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma.; Authors - Zhenyuan Han
mouse	WB,IF	Mol Brain; Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.; Authors - Peipei Chen ; KO Validated
human	WB	Cell Biochem Biophys; NSUN5-FTH1 Axis Inhibits Ferroptosis to Promote the Growth of Gastric Cancer Cells; Authors - Yibin Su ; KD Validated