NSDHL Polyklonaler Antikörper

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• KD/KO Validated

NSDHL Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15111-1-AP

Synonyme

H105E3, NSDHL, Protein H105e3, SDR31E1, XAP104

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15111-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15111-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15111-1-AP (NSDHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 15111-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 15111-1-AP (NSDHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 15111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15111-1-AP (NSDHL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Eierstockgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lungengewebe, humanes Lungenkarzinomgewebe, humanes Magengewebe, humanes Magenkrebsgewebe, humanes Ovarialkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3200
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below

Produktinformation

15111-1-AP bindet in WB, IHC, IF, ELISA NSDHL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NSDHL fusion protein Ag7097
• Vollständiger Name: NAD(P) dependent steroid dehydrogenase-like
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 40 kDa
• GenBank-Zugangsnummer: BC000245
• Gene symbol: NSDHL
• Gene ID (NCBI): 50814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NSDHL(Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating) encodes a sterol dehydrogenase or decarboxylase involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis. Mutations in this gene are associated with human CHILD syndrome (congenital hemidysplasia with ichthyosiform nevus and limb defects), an X-linked, male lethal disorder, as well as the mouse mutations bare patches and striated.(PMID: 14506130).

Publikationen

Species	Application	Title
mouse	WB	Cancer Cell; Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer.; Authors - Linara Gabitova-Cornell ; KO Validated
mouse	IHC	Cancer Discov; Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGF receptor inhibitors via increased EGF receptor degradation.; Authors - Sukhanova Anna A
mouse	WB,IHC	Clin Cancer Res; Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma.; Authors - Renata E Gordon ; KD Validated
mouse	WB, IHC	Cell Rep; Endogenous Sterol Metabolites Regulate Growth of EGFR/KRAS-Dependent Tumors via LXR.; Authors - Linara Gabitova
mouse	IHC	Cell Cycle; Insm2 deficiency results in female infertility by disturbing steroid pathway and decreasing ovarian reserve in mice.; Authors - Zhi-Ming Li
human	IHC	Mol Cancer Ther; EGFR and RB1 as Dual Biomarkers in HPV-Negative Head and Neck Cancer.; Authors - Tim N Beck